This document identifies parameters and conditions, as part of an integrated measurement system,necessary to develop and validate methods for the application of asymmetrical-flow and centrifugalfield-flow fractionation to the analysis of nano-objects and their aggregates and agglomerates dispersedin aqueous media. In addition to constituent fractionation, analysis can include size, size distribution,concentration and material identification using one or more suitable detectors. General guidelines andprocedures are provided for application, and minimal reporting requirements necessary to reproduce amethod and to convey critical aspects are specified.
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