BS PD ISO/TS 18220:2016 specifies an early-life stage procedure for determination of the toxiceffects of chemicals and water samples on a cold-water brackish water copepod species under semistaticconditions. The biological test variables include survival and development of the early-life stages.The exposure starts with newly hatched (<24 h) nauplii (larvae) and is continued until emergence of(c. 50 %) copepodites (juveniles) in the control.The benthic living Nitocra complements the planktonic Acartia species in ISO 16778. These organismsrepresent different life-history strategies as Nitocra is egg-carrying, whereas Acartia is a broadcastingcalanoid copepod and thus, different sensitivities of specific life stages. Nitocra is a fresh to brackishwater species, which allows testing low salinity waters and is complementary to A. tonsa, which is ofmarine origin and poorly tolerates low salinities.Cross References:ISO 5667-16ISO 14669ISO 16778ISO/TS 20281SS 28106
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