ASTM D4455-85(2014)

1.1This test method describes a procedure for detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure. It is applicable to environmental waters.

1.2Certain types of debris and other microorganisms may fluoresce in acridine orange-stained smears.

1.3The test method requires a trained microbiologist or technician who is capable of distinguishing bacteria from other fluorescing bodies on the basis of morphology when viewed at higher magnifications.²

1.4Use of bright light permits differentiation of single bacteria where reduced formazan is deposited at the polar ends.

1.5Approximately 10⁴ cells/mL are required for detection by this test method.²

1.6The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.7This standard does not purport to address the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.