BS PD ISO/TS 21569-3:2020

This document describes a procedure for the detection of the DNA transition sequence between the 35Spromotor (P35S) from Cauliflower mosaic virus and a modified phoshinothricin-acetyltransferase gene(pat) from Streptomyces viridochromogenes. The P35S-pat construct is frequently found in genetically modified plants with tolerance for phosphinothricin-containing herbicides. The P35S-pat constructspecific method is based on a real-time PCR and can be used for qualitative and quantitative screeningpurposes. For identification and quantification of a specific event, a follow-up analysis can be carried out.